General Cell Based Assays
Innovative Research Inc Hmn uPA Rcmb LMW 100ug
Human Urokinase (uPA) Recombinant Low Molecular Weight 100ug - Human Urokinase (uPA) Recombinant Low Molecular Weight from Innovative Research has been recombinantly produced in insect cells and purified using a combination of conventional and affinity chromatography. This is a frozen liquid buffered in 0.05M Sodium Acetate; 0.1M NaCl; 1mM EDTA; pH 5.0 and a purity of 95%. It is useful as a positive control for ELISA and western blot and and other applications including cell differentiation, cell signaling, clotting assays, fibrinolysis, plasminogen activation, protein-protein interactions, receptor binding, thromboelastography, and thrombolysis. Purified from auto-catalytic digestion of recombinant human urokinase produced in insect cells. Active two-chain low molecular weight human urokinase contains the proteinase domain and is separated from the amino terminal fragment by cleavage at the Lys135-Lys136 bond.
Cayman Chemical D-Fructose-1 6-bIsphosphate 5g
An intermediate in carbohydrate metabolism, including glycolysis and gluconeogenesis
Apexbio Technology LLC Red Fluorometric Lipid Droplet Assay Kit 100T
Red Fluorometric Lipid Droplet Assay Kit is a fluorescent detection kit designed for the visualization and quantification of intracellular lipid droplets It is intended to probe and monitor lipid metabolism by specifically labeling intracellular neutral lipid accumulations This kit exerts its function primarily through the use of Nile Red a cell-permeable lipophilic dye that selectively accumulates within neutral lipid environments resulting in red fluorescence upon excitation (552 nm emission 636 nm) No key metric (IC50/EC50) or specific cell line information is available Based on these properties Red Fluorometric Lipid Droplet Assay Kit enables research applications in cellular lipid metabolism autophagy cell differentiation and senescence
Hach Company SC4500 Ctrl Prog Prof DP 1 mA
SC4500 Controller-Prognosys-Profibus DP-1 mA Input-100-240 VAC-without power cordIncludes Controller-Non-Claros Embedded Prognosys-without power cord includes mounting hardware
Eurofins Discoverx PathHunter eXpress BMPR1B/ACVR2A Dimerization Assay, 10pl Kit. (93-1010E3CP0L)
The PathHunter BMPR1B/ACVR2A Dimerization eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting receptor-receptor interaction. This kit assesses ligand (e.g. BMP-6) induced dimerization of BMPR1B/ACVR2A receptors. This product uses EFC technology. The cell line included overexpresses BMPR1B target protein fused to PK alongside ACVR2A target protein fused to EA. Upon ligand-induced dimerization of BMPR1B/ACVR2A, detectable EFC signal is produced. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx PathHunter eXpress rGPR120 CHO-K1 β-Arrestin GPCR Assay, 10pl Kit. (93-1031E2RCP0L)
The PathHunter rGPR120 eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. free fatty acids) based activation of rGPR120 GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged rGPR120 and EA-tagged β-Arrestin-2. Activation of rGPR120 stimulates the recruitment of β-Arrestin-2 and produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. Please note that this product expresses the Rat version of this protein. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Cayman Chemical SupRoxID DIsmutase asy KI 96
Cayman’s Superoxide Dismutase Assay Kit provides a convenient method of measuring SOD activity in plasma, serum, erythrocyte lysates, tissue homogenates, and cell lysates. The assay relies on xanthine oxidase to produce superoxide radicals, which then reduce a tetrazolium salt to a formazan dye, detectable at 450 nm. SOD in the sample inhibits this reduction by catalyzing the dismutation of superoxide radicals. Thus, the degree of inhibition is directly proportional to SOD activity. The SOD Assay Kit measures all three types of SOD (Cu/Zn-, Mn-, and Fe-SOD). Mitochondrial Mn-SOD can be assayed separately following the procedure outlined in the kit booklet.
Cell Biolabs Inc ViraDuctin™ AAV Transduction Kit, 50 transductions
AAV can infect both dividing and non-dividing cells and can be maintained in the human host cell, creating the potential for genome integration. Because AAV is a naturally defective virus, requiring provision of several factors in trans for productive infection, it is considered the safest viral vector to use. The AAV transduction process includes viral binding and entry, intracellular trafficking, nuclear transport, and viral second strand DNA synthesis. The viral second strand DNA synthesis has been shown to be the rate limiting step, which leads to inefficient transduction by AAV vectors. ViraDuctin™ AAV Transduction Kit is a proprietary formulation designed to increase the transduction efficiency of AAV vectors in a variety of cell types. This kit provides the following advantages: (1) Higher transduction efficiency in many cell types, (2) Easy to use, and (3) It is ideal for transduction of nonpermissive cells such as primary cells and stem cells. 50 transductions/kit.
Sigma Aldrich Fine Chemicals Biosciences Proteasome 20S Activity As
Protein degradation occurs most commonly through two major mechanisms lysosomal degradation and proteasome mediated degradation. Lysosomal degradation occurs through proteolytic enzyme activity and is fairly non-specific. Proteasomal degradation is targeted for a specific protein through the ubiquitination of the target protein. The target protein is typically poly-ubiquitinated and activates the 26S proteasome toward proteolysis. The proteasome 26S is the most common form of the proteasome and is an ATP-dependent proteolytic complex which contains one 20S (700-kDa) core particle structure and two 19S (700-kDa) regulatory caps.The proteasome is responsible for the recycling of proteins and maintenance of the balance of protein synthesis and degradation. The proteasome is involved with apoptosis DNA repair endocytosis and cell cycle and has been implicated in disease states such as certain types of cancer diabetes mellitus and Alzheimer disease among others making it the target f
Cayman Chemical Glucose Uptake CelBased As 1ea
Cayman’s Glucose Uptake Cell-Based Assay Kit provides a convenient tool for studying modulators of cellular glucose uptake. The kit employs NBD-glucose (2-NBDG), a fluorescently labeled deoxyglucose analog, as a probe for the detection of glucose taken up by cultured cells. Researchers interested in cellular metabolism, such as cancer biologists, immunologists, physiologists, and others, will find this kit a robust tool for measuring energy consumption in living cells.
Eurofins Discoverx PathHunter Human FGFR4-alpha-Klotho Functional Kinase Cell-Based Assay Kit (U2OS)
The PathHunter Human FGFR4-alpha-Klotho Stable Cell Line Functional Kinase Assay (U2OS) measures FGFR4-α-Klotho receptor tyrosine kinase activity via ligand-induced SH2 domain protein recruitment. This is a complete kit that includes the cell vials and all required reagents needed for cell culture and running the assay.
Meso Scale Discovery U-PLEX Mouse IL-9 ASY
The Meso Scale Discovery U-PLEX Mouse IL-9 Assay Kit is a multiplex immunoassay that measures mouse interleukin 9 (IL-9) in serum plasma and cell culture supernatants It uses electrochemiluminescence (ECL) detection technology and provides high sensitivity up to 5 logs of dynamic range and low sample volume requirements This assay kit includes a 96-well plate coated with capture antibodies detection antibodies labeled with an ECL tag calibrators and diluents It can accurately quantify IL-9 concentrations from as low as 24 pg/mL to 50 000 pg/mL The assay takes 3 5 hours to complete including a 2 hour incubation step
Eurofins Discoverx PathHunter Human IL-18 (IL18R1/IL18RAP) Dimerization Cell-Based Assay Kit (U2OS)
The PathHunter Human IL-18 (IL18R1/IL18RAP) Stable Cell Line Dimerization Assay (U2OS) measures ligand-induced dimerization of IL18R1/IL18RAP. This is a complete kit that includes the cell vials and all required reagents needed for cell culture and running the assay.
American Research Products Inc HOXA11/D11 Transcription Factor Activity Assay
HOXA11/D11 Transcription Factor Activity Assay
Cayman Chemical LIpIdLaunchALC0315 LNP KIt 1ea
Cayman’s LipidLaunch™ ALC-0315 LNP Kit (Loadable) provides cargo-ready, empty ALC-0315 LNPs. These LNPs are capable of rapid encapsulation of nucleic acids, without the need for incubation steps, enabling subsequent delivery to target cells or other downstream research applications. LipidLaunch™ LNPs (Loadable) demonstrate very low toxicity compared to traditional lipid-based transfection methods. Conventionally loaded LNPs typically require specialized equipment to ensure consistent control of particle size, while also requiring large reaction volumes. LipidLaunch™ ALC-0315 LNPs (Loadable) enable users to encapsulate cargo in low reaction volumes offering greater flexibility in the scale of preparation while preserving costly nucleic acid cargo.